Ruchi Bajpai

CSA 207D, HSC 9062
Phone: (323)442-1220
Fax: (323)442-2981
rbajpai@usc.edu
website:

Ruchi Bajpai, Ph.D.

Assistant Professor , Ostrow School of Dentistry of USC, University of Southern California, Los Angeles, CA

Assistant Professor , Keck School of Medicine, University of Southern California, Los Angeles, CA

  • North Eastern Hill University, Shillong, India
    B.S.
    1994
    Biological Sciences

  • North Eastern Hill University, Shillong, India
    M.S.
    1996
    Biochemistry

  • Jawaherlal Nehru University, New Delhi, India
    Ph.D.
    2003
    Developmental Genetics

  • 2011 - present - Assistant Professor - Ostrow School of Dentistry of USC, University of Southern California, Los Angeles, CA
  • 2011 - present - Assistant Professor - Keck School of Medicine, University of Southern California, Los Angeles, CA
  • 2010 - 2011 - Research Associate - Wysocka lab, Stanford University, CA
  • 2007 - 2010 - Postdoctoral Scholar - Wysocka lab, Stanford University, CA
  • 2002 - 2006 - Postdoctoral Fellow - Terskikh lab, The Burnham Institute, CA
  • 1997 - 2003 - Graduate Student - Centre for Cellular and Molecular Biology, Jawaherlal Nehru University, New Delhi, India
  • 2013 - Invited Seminar Speaker at a premier undergraduate training and research Institute at ISSER Pune India. Feb: 2013
  • 2013 - Invited Speaker at a premier Stem cell research Institute at Bangalore India ( Instem/ affiliate of Tata Institute of Fundamental research): Jan 2013
  • 2013 - Invited Seminar at Scripps Research Institute, La Jolla, CA; April 2013
  • 2013 - Invited Speaker at Next Generation Stem Cell Research Conference, Albany, May 8-9, 2013
  • 2013 - Invited Speaker at Gordon Research Conference for Neural Crest and Placodes, Jul 2013
  • 2012 - Speaker at the USCF-UCLA-USC Tri-institutional stem cell retreat held at Asilomar, Mar 2012
  • 2012 - Selected Speaker at the Gordon Research Conference on Craniofacial Morphogenesis and Tissue Regeneration, Ventura, CA, March 2012.
  • 2012 - Invited Seminar Speaker, Department of Human Genetics, Leiden University Medical Center,
  • 2010 - Selected Speaker at Gordon Research Seminar on Craniofacial Morphogenesis and Tissue Regeneration, Barga, Italy, April 2010.
  • 2010 - Invited Speaker at the Northern California Clinical Genetics Exchange, Lucile Packard Children’s Hospital, Stanford University, CA; May 2010
  • 2010 - Invited Speaker at the Biochemistry Department, NEHU, Shillong India; Oct 2010
  • 2010 - Invited Speaker at the Institute for Genomics and Integrative Biology, India; Nov 2010
  • 2006 - Postdoctoral fellowship from California Institute for regenerative medicine
  • 2005 - Fishman award for Postdoctoral Career Development
  • 2001 - Travel grant from the company of biologists (Development) for visiting EMBL, Germany.
  • 2001 - Short-term fellowship award from MCBN-UNESCO to visit Dr. Steve Cohen’s lab at EMBL, Germany.
  • 2000-2002 - Senior Research Fellowship from Council for Scientific and Industrial Research, India
  • 1997-2000 - Junior Research Fellowship from Council for Scientific and Industrial Research, India
  • 1994-1996 - Merit Scholarship, MS
  • 1991-1994 - Merit Scholarship, BS

Epigenetic profile based identification of regulatory circuitry to determinine diagnostic and prognostic targets in Neuroblastomas - Grant #

  • Principal Investigator: Ruchi Bajpai, Ph.D.
  • Sponsor: SC CTSI (NIH/NCRR/NCATS) through Grant UL1TR000130
  • Period: 07/01/2013-06/30/2014
  • Goal: This project is aimed at determining regulatory regions that are uniquely activated in subsets of cells within heterogeneous neuroblastomas.

Eoigenetic annotation of Neuroblastoma regulatory elements to develop novel prognostic and diagnostic tools - Grant #IRG 58-007-54

  • Principal Investigator: Ruchi Bajpai, Ph.D.
  • Sponsor: American Cancer Society
  • Period: 03/01/2013-02/28/2014
  • Goal: This project is aimed at determining regulatory regions that are actively driving gene expression in peripheral nervous system derived pediatric tumors called neuroblastomas.

Epigenetic Control of Human Neural Crest Formation: Impact on Neurocristopathie - Grant #5 R01 DE024584

  • Principal Investigator: Ruchi Bajpai, Ph.D.
  • Sponsor: National Institute of Dental and Craniofacial Research, NIH
  • Period: 07/01/2014-03/31/2019
  • Goal: Defects in the development of neural crest-derived tissues are responsible for some of the most common birth defects many of which present with characteristic facial features. Here we propose to investigate CHD7 and PHF6, proteins that are defective in two such syndromes with contrasting features-CHARGE and Börjeson-Forssman-Lehmann syndromes respectively using Xenopus and human stem cells as model systems for our studies.

Identifying negative regulators of CHD7 as potential therapeutic targets for CHARGE - Grant #2014 Pilot Grant

  • Principal Investigator: Ruchi Bajpai, Ph.D.
  • Sponsor: Charge Syndrome Foundation, Inc.
  • Period: 07/01/2014-06/30/2015
  • Goal: This project will develop Zebrafish genetic model for studying CHARGE and BFLS syndromes and assess the impact of recreating patient mutations on embryonic craniofacial development.
  1. Asad Z, Pandey A, Babu A, Sun Y, Shevade K, Kapoor S, Ullah I, Ranjan S, Scaria V, Bajpai R, Sachidanandan C. (2016) Rescue of neural crest-derived phenotypes in a zebrafish CHARGE model by Sox10 downregulation. Hum Mol Genet. 2016 Aug 15;25(16):3539-3554. doi: 10.1093/hmg/ddw198. PubMed PMID: 27418670; PubMed Central PMCID: PMC5179949.

  2. Kerosuo L, Nie S, Bajpai R, Bronner ME. (2015) Crestospheres: Long-Term Maintenance of Multipotent, Premigratory Neural Crest Stem Cells. Stem Cell Reports. 2015 Oct 13;5(4):499-507. doi: 10.1016/j.stemcr.2015.08.017. PubMed PMID: 26441305; PubMed Central PMCID: PMC4625028.

  3. Rada-Iglesias A, Bajpai R, Prescott S, Brugmann SA, Swigut T, Wysocka J. (2012) Epigenomic annotation of enhancers predicts transcriptional regulators of human neural crest. Cell Stem Cell. 2012 Nov 2;11(5):633-48. doi:10.1016/j.stem.2012.07.006. Epub 2012 Sep 13. PubMed PMID: 22981823; PubMed Central PMCID: PMC3751405.

  4. Crocker SJ, Bajpai R, Moore CS, Frausto RF, Brown GD, Pagarigan RR, Whitton JL, Terskikh AV. (2011) Intravenous Administration of Human ES-derived Neural Precursor Cells Attenuates Cuprizone-induced CNS Demyelination. Neuropathol Appl Neurobiol. 2011 Oct;37(6):643-53. doi:10.1111/j.1365-2990.2011.01165.x. PubMed PMID: 21276029; PubMed Central PMCID:PMC3252213.

  5. Bajpai R. (2011) Lentivirus-mediated modification of pluripotent stem cells. Methods Mol Biol. 2011;767:315-31. doi: 10.1007/978-1-61779-201-4_23. PubMed PMID: 21822885.

  6. Rada-Iglesias A, Bajpai R, Swigut T, Brugmann SA, Flynn RA, Wysocka J. (2011) A unique chromatin signature uncovers early developmental enhancers in humans. Nature. 2011 Feb 10;470(7333):279-83. doi: 10.1038/nature09692. PubMed PMID: 21160473; PubMed Central PMCID: PMC4445674.

  7. Cordero DR, Brugmann S, Chu Y, Bajpai R, Jame M, Helms JA. (2011) Cranial neural crest cells on the move: their roles in craniofacial development. Am J Med Genet A. 2011 Feb;155(2):270-9. Review. PubMed PMID: 21271641; PubMed Central PMCID: PMC3039913.

  8. Bajpai R, Chen DA, Rada-Iglesias A, Zhang J, Xiong Y, Helms J, Chang CP, Zhao Y, Swigut T, Wysocka J. (2010) CHD7 cooperates with PBAF to control multipotent neural crest formation. Nature. 2010 Feb 18;463(7283):958-62. Epub 2010 Feb 3. PubMed PMID: 20130577; PubMed Central PMCID: PMC2890258.

  9. Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot JG, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch AD, Price JH, Conklin BR, Chen HS, Mercola M. (2009) Lentiviral vectors and protocols for creation of stable hESC lines for fluorescent tracking and drug resistance selection of cardiomyocytes. PLoS One. 2009;4(4):e5046. Epub 2009 Apr 8. PubMed PMID: 19352491; PubMed Central PMCID: PMC2662416.

  10. Bajpai R, Coppola G, Kaul M, Talantova M, Cimadamore F, Nilbratt M, Geschwind DH, Lipton SA, Terskikh AV. (2009) Molecular stages of rapid and uniform neuralization of human embryonic stem cells. Cell Death Differ. 2009 Jun;16(6):807-25. doi:10.1038/cdd.2009.18. PubMed PMID: 19282867; PubMed Central PMCID: PMC3432273.

  11. Jaksch M, Múnera J, Bajpai R, Terskikh A, Oshima RG. (2008) Cell cycle-dependent variation of a CD133 epitope in human embryonic stem cell, colon cancer, and melanoma cell lines. Cancer Res. 2008 Oct 1;68(19):7882-6. PubMed PMID: 18829544; PubMed Central PMCID: PMC2561223.

  12. Nakano I, Masterman-Smith M, Saigusa K, Paucar AA, Horvath S, Shoemaker L, Watanabe M, Negro A, Bajpai R, Howes A, Lelievre V, Waschek JA, Lazareff JA, Freije WA, Liau LM, Gilbertson RJ, Cloughesy TF, Geschwind DH, Nelson SF, Mischel PS, Terskikh AV, Kornblum HI. (2008) Maternal embryonic leucine zipper kinase is a key regulator of the proliferation of malignant brain tumors, including brain tumor stem cells. J Neurosci Res. 2008 Jan;86(1):48-60. PubMed PMID: 17722061.

  13. Bajpai R, Lesperance J, Kim M, Terskikh AV. (2008) Efficient propagation of single cells Accutase-dissociated human embryonic stem cells. Mol Reprod Dev. 2008 May;75(5):818-27. PubMed PMID: 18157870.

  14. Bruey JM, Bruey-Sedano N, Luciano F, Zhai D, Balpai R, Xu C, Kress CL, Bailly-Maitre B, Li X, Osterman A, Matsuzawa S, Terskikh AV, Faustin B, Reed JC. (2007) Bcl-2 and Bcl-XL regulate proinflammatory caspase-1 activation by interaction with NALP1. Cell. 2007 Apr 6;129(1):45-56. PubMed PMID: 17418785.

  15. Katkov II, Kim MS, Bajpai R, Altman YS, Mercola M, Loring JF, Terskikh AV, Snyder EY, Levine F. (2006) Cryopreservation by slow cooling with DMSO diminished production of Oct-4 pluripotency marker in human embryonic stem cells. Cryobiology. 2006 Oct;53(2):194-205. Epub 2006 Jul 12. PubMed PMID: 16839540.

  16. Nakano I, Paucar AA, Bajpai R, Dougherty JD, Zewail A, Kelly TK, Kim KJ, Ou J, Groszer M, Imura T, Freije WA, Nelson SF, Sofroniew MV, Wu H, Liu X, Terskikh AV, Geschwind DH, Kornblum HI. (2005) Maternal embryonic leucine zipper kinase (MELK) regulates multipotent neural progenitor proliferation. J Cell Biol. 2005 Aug 1;170(3):413-27. PubMed PMID: 16061694; PubMed Central PMCID: PMC2171475.

  17. Bajpai R, Sambrani N, Stadelmayer B, Shashidhara LS. (2004) Identification of a novel target of D/V signaling in Drosophila wing disc: Wg-independent function of the organizer. Gene Expr Patterns. 2004 Nov;5(1):113-21. PubMed PMID: 15533826.

  18. Bajpai R, Makhijani K, Rao PR, Shashidhara LS. (2004) Drosophila Twins regulates Armadillo levels in response to Wg/Wnt signal. Development. 2004 Mar;131(5):1007-16. PubMed PMID: 14973271.

  19. Bharathi V, Pallavi SK, Bajpai R, Emerald BS, Shashidhara LS. (2004) Genetic characterization of the Drosophila homologue of coronin. J Cell Sci. 2004 Apr 15;117(Pt 10):1911-22. PubMed PMID: 15090595.

  20. Prasad M, Bajpai R, Shashidhara LS. (2003) Regulation of Wingless and Vestigial expression in wing and haltere discs of Drosophila. Development. 2003 Apr;130(8):1537-47. PubMed PMID: 12620980.

  21. Shashidhara LS, Agrawal N, Bajpai R, Bharathi V, Sinha P. (1999) Negative regulation of dorsoventral signaling by the homeotic gene Ultrabithorax during haltere development in Drosophila. Dev Biol. 1999 Aug 15;212(2):491-502. PubMed PMID:10433837.